Composite
PCA system

Part:BBa_K5291031:Design

Designed by: Rui Chen   Group: iGEM24_BNUZH-China   (2024-09-30)


pAB1-pS-PAO102


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 110
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 110
    Illegal NotI site found at 291
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 110
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 110
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have known from papers related to PE degradation that during this process a huge amount of CO2 will be released. So we introduce PCA system into Pseudomonas aeruginosa to address the excessive of CO2. We choose PAO102 native in P. aeruginosa as well as pS and BiTerm usable in the engineered bacteria to secure the expression of the system.

Source

pS, BiTerm: Escherichia coli
PAO102: Pseudomonas aeruginosa

References

[1]Chen YJ, Liu P, Nielsen AA, Brophy JA, Clancy K, Peterson T, Voigt CA. Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods. 2013 Jul;10(7):659-64. doi: 10.1038/nmeth.2515. Epub 2013 Jun 2. PMID: 23727987.
[2]Lotlikar, S.R., et al., Three functional beta-carbonic anhydrases in Pseudomonas aeruginosa PAO1: role in survival in ambient air. Microbiology (Reading), 2013. 159(Pt 8): p. 1748-1759.
[3]Thorbecke, R., et al., The gene dosage effect of carbonic anhydrase on the biosynthesis of poly ( 3-hydroxybutyrate ) under autotrophic and mixotrophic culture conditions. POLYMER JOURNAL, 2021. 53(1): p. 209-213.